LOCUS       40924_25946        6329 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJBS633 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Okamoto S, Niki H.
  TITLE     cloning vector pJBS633 sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory, Genetics Strains Research 
            Center; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/
REFERENCE   3  (bases 1 to 6329)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2016) Contact:Sho Okamoto National Institute of Genetics, 
            Microbial Genetics Laboratory, Genetics Strains Research Center; 
            1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(103..127)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      552..979
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1289..2101)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2223..2811
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3433..4290)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(5066..6253)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(6301..6329)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa
       61 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
      121 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
      181 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
      241 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
      301 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
      361 gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
      421 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
      481 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
      541 ggaaataata aagcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      601 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      661 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      721 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      781 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      841 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      901 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      961 caaaaattta acgcgaatta cgccctgata gacggttttt cgccctttga cgttggagtc
     1021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1141 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcaggtgg
     1201 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1261 tatgtatccg ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca
     1321 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     1381 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     1441 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     1501 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     1561 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     1621 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     1681 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     1741 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     1801 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     1861 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     1921 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     1981 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     2041 catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca
     2101 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc
     2161 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2221 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2281 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2341 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2401 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2461 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2581 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2641 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2821 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2881 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2941 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3001 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc
     3061 tcagtacaat ctgctctgat gccgcatagt taagcccagt aaggcaaccc cgccagccta
     3121 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc
     3181 ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
     3241 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
     3301 acatgagaat tctggttggc agcgtttaaa agatcgtaaa gaatttaaat ccgttttggg
     3361 ttggatacgt tcaacagaag taacaaaagg aaaagacgga tctgcacatc ctcattttca
     3421 tattttgctt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3481 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3541 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3601 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3661 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3721 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     3781 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3841 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3901 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3961 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4021 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     4081 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4141 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4201 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4261 aagggcgaca cggaaatgtt gaatactcat atggcccact acgtgaacca tcaccctaat
     4321 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4381 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4441 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4501 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccacgccgg catggcggcc
     4561 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
     4621 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
     4681 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
     4741 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
     4801 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
     4861 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
     4921 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
     4981 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaatccgtta
     5041 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
     5101 acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg
     5161 tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccaatgatc gaagttaggc
     5221 tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
     5281 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     5341 ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg
     5401 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     5461 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     5521 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     5581 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     5641 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat
     5701 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     5761 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     5821 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     5881 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     5941 cgatgcgtcc ggcgtagaga atccacagga cgggtgtggt cgccatgatc gcgtagtcga
     6001 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
     6061 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
     6121 agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca
     6181 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
     6241 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
     6301 attaaagctt atcgatgata agctgtcaa
//
